APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr286 of human CaMKII alpha. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
calcium/calmodulin dependent protein kinase II alpha , CAMKA , CaMKIINalpha , MRD53 , MRT63
Cellular Localization
Cell junction, synapse,Postsynaptic density,Cell projection, dendritic spine,Cell projection, dendrite
Background
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018]
Database
Research Area
DATA IMAGES
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GTX640251 ICC/IF Image
CaMKII (phospho Thr286) antibody [HL2908] detects CaMKII (phospho Thr286) protein by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.Green: CaMKII (phospho Thr286) stained by CaMKII (phospho Thr286) antibody [HL2908] (GTX640251) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX640251 WB Image
Untreated (–) and treated (+) mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII (phospho Thr286) antibody [HL2908] (GTX640251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.(CIP: Calf-intestinal alkaline phosphatase)
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REFERENCE
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REVIEW
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